Figure 2. Laser scanning confocal microscopy results used to investigate double labeling of α-sarcomeric actin and cTnT proteins in rat bone marrow mesenchymal stem cells (rBMSCs) treated with 5 ng/mL TGF-β1, evaluated by fluorescence. A, Red light indicating α-sarcomeric actin; B, green light indicating cTnT; C, the presence of both proteins was indicated by yellow light; D, bar graphs showing the double labeling of α-sarcomeric actin and cTnT of rBMSCs treated with different concentrations of TGF-β1. Magnification bar: 25 μm. Data are reported as means±SD. *P<0.05 vs 2 ng/mL group (one-way ANOVA).