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. Author manuscript; available in PMC: 2016 Jul 5.
Published in final edited form as: Biochemistry. 2016 May 19;55(22):3102–3106. doi: 10.1021/acs.biochem.6b00214

Figure 1.

Figure 1

Schematic representation of the experimental setup. Protein is covalently attached to the AFM probe via a PEG tether (red), and ssDNA (green) is covalently attached at the 5′ end to the functionalized mica surface. (A) Initial position, where A3A and ssDNA are far from forming a complex. (B) As the probe approaches the surface, A3A captures ssDNA and forms a complex. (C) Retraction step causing stretching of the PEG tether and ssDNA. (D) Rupture of the complex.