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. Author manuscript; available in PMC: 2016 Jul 5.
Published in final edited form as: Bio Protoc. 2014 Mar 20;4(6):e1079. doi: 10.21769/bioprotoc.1079
Stock 20 ml Final conc.
1M Tris-Cl [pH 8.0] 200 µl 10 mM
5M NaCl 1600 µl 400 mM
0.1 M PMSF 40 µl 0.2 mM
1 mg/ml Aprotinin 20 µl 1 µg/ml
1 mg/ml Leupeptin 20 µl 1 µg/ml
1 mg/ml Pepstatin A 20 µl 1 µg/ml
ddH2O to 20 ml