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. 2016 Jun 14;113(26):7183–7188. doi: 10.1073/pnas.1522381113

Fig. 2.

Fig. 2.

dhhc10 is translationally repressed in gametocytes and translated in ookinetes, where it localizes to the crystalloid. (A) Only dhhc10::gfp mRNA is transcribed in dhhc10::gfp parasites. p28 served as the control gene for RT-PCR. (B) DHHC10::GFP is translated only in ookinetes. DNA was stained with Hoechst 33342. (Scale bar: 5 µm.) (C) RT-PCR analyses of DOZI::GFP and CITH::GFP gametocyte IP eluates showing that dhhc10 mRNA, like p25 and p28 mRNAs, is bound by both translational repressors. Translated mRNAs (dozi, cith, and alba-3) are not enriched in the IP-GFP fractions. Input, total gametocyte mRNA; IP-GFP, IP with anti-GFP antibody; IP-c-myc, IP with anti–c-myc antibody; IP beads, no antibody used for IP. (D) DHHC10::GFP colocalizes with the crystalloid-resident protein LAP3 (here tagged with mCherry). (Inset) A higher-magnification view of the crystalloid as seen on DIC microscopy. N, nucleus stained with Hoechst 33342. (Scale bar: 5 µm.)