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. 2016 Jun 6;113(26):7118–7123. doi: 10.1073/pnas.1525184113

Fig. 1.

Fig. 1.

Functional analysis of OsNRT2.3b, OsNRT2.3a + OsNAR2.1, and H167R transporters expressed in Xenopus oocytes. (A–C) Oocyte plasma membrane potential changes in response to 1 mM nitrate treatment (shaded bar) and pH 8.0 saline wash (black bar) for a cell expressing OsNRT2.3b (A), OsNRT2.3a + OsNAR2.1 (B), and OsNRT2.3b-H167R mutant (C). (D–F) Double-barreled pH electrode recording of cytosolic pH from oocytes injected with OsNRT2.3b treated with 1 mM nitrate (shaded bar) and pH 8.0 saline (black bar) washing (D), with OsNRT2.3a + OsNAR2.1 (E), and with OsNRT2.3b-H167R (F) treated with 1 mM nitrate (shaded bar).