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FKBP65 does not direct LH2 toward procollagen Iα1. Bright-field PLA for LH2 and procollagen-Iα1 or control PLA for LH2 and CD31 in NHDFs, treated with FKBP65 PPIase inhibitor tacrolimus or DMSO control combined with or without TGFβ1 treatment for 24 h (A), or transfected with esiRNA against FKBP10 or controls (RLUC and PLOD2), followed by TGFβ1 treatment for 2 d (B). Arrows indicate examples of PLA signals from LH2 and procollagen Iα1. (Scale bars: 50 µm.)
