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. 2016 Jun 15;113(26):7219–7224. doi: 10.1073/pnas.1600581113

Fig. 3.

Fig. 3.

Plasma IGF1 levels and IGF1 secretion in primary cells from the TBC1D1 KI mice. (A) Plasma levels of IGF1 in the male WT and KI mice (8 wk old). n = 7. (B) Colocalization of TBC1D1 and IGF1 in primary hepatocytes. Bars indicate 10 μm in length. (C and D) Expression of TBC1D1 and IGF1 in TBC1D1-depleted primary hepatocytes via siRNA (C) or in primary hepatocytes from the TBC1D1 KO mice (D). NC, negative control. (E and F) IGF1 secretion in TBC1D1-depleted primary hepatocytes (E) or in primary hepatocytes from the TBC1D1 KO mice (F). NC, negative control. n = 6. (G) IGF1 secretion in primary hepatocytes from the WT and TBC1D1 KI mice. aP < 0.05 (WT metformin or AICAR vs. WT untreated) and bP < 0.05 (KI metformin or AICAR vs. KI untreated). n = 8. (H) IGF1 secretion in primary adipocytes from the KI mice. WT: n = 5; KI: n = 6. The data are given as the mean ± SEM, and the asterisk indicates P < 0.05. Statistical analysis for G was carried out using two-way ANOVA.