Fig. 2.

Structure of the C. albicans Tps2NTD and functional importance of Tps2. (A) Cartoon diagram of the Tps2NTD. The N-terminal and C-terminal Rossmann-fold domains are depicted as ribbons and colored cyan and pink, whereas the C-terminal helix that interacts with both domains is colored red. (B) The phosphatase domain of Tps2 is sufficient to rescue the temperature-sensitive phenotype of tps2∆ in C. neoformans at 37 °C but not at 39 °C, whereas altering a single amino acid residue (D705N), predicted to eliminate Tps2 activity, failed to restore growth at elevated temperatures. The strains were incubated for 3 d.