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. 2016 Jun 15;113(26):7148–7153. doi: 10.1073/pnas.1601774113

Fig. 5.

Fig. 5.

Structures Tps2PD in the open conformation and the catalytic cycle of Tps2. (A) Side view of A. fumigatus Tps2PD structures in the open conformation from three different crystal forms. The Tps2PD structures are depicted as cartoon and displayed after the superposition of their respective core domains. Crystal forms 1, 2, and 3 are colored in cyan, pink, and wheat, respectively. (B) “Top” view of the superposition of the three A. fumigatus Tps2PD structures. (C) Structural superposition of the core domains of A. fumigatus Tps2PD crystal form 2 and the C. albicans Tps2PD transition-state complex. The yellow dots depict the locations of the Cα atom of residue E123 in the closed and open conformations. (D) The Tps2PD catalytic cycle requires conformational changes: Open/apo enzyme ⇒ closed/substrate bound ⇒ transition state/aspartylphosphate formation ⇒ open/product release effected by substrate binding, catalysis, and product release.