Table S1.
Identification of proteins associated with EX1 by coimmunoprecipitation and mass spectrometry
| AGI code | Protein | No. of peptide matches | Sequence coverage, % | ||||||||
| 1 | 2 | 3 | 4 | BC | DC | 1 | 2 | 3 | 4 | ||
| AT4G33630 | EX1 | protein of unknown function (DUF3506) | 68 | 55 | 44 | 41 | 0 | 0 | 65.6 | 65.4 | 66.5 | 53 |
| ATCG00020 | PSBA | photosystem II reaction center protein A | 12 | 3 | 11 | 5 | 3 | 2 | 13.3 | 11.1 | 44.2 | 16 |
| ATCG00680 | PSBB | photosystem II reaction center protein B | 22 | 22 | 20 | 12 | 5 | 7 | 39.8 | 29.1 | 39.8 | 23 |
| ATCG00280 | PSBC | photosystem II reaction center protein C | 19 | 19 | 19 | 10 | 4 | 3 | 27.3 | 29.4 | 27.3 | 27 |
| ATCG00270 | PSBD | photosystem II reaction center protein D | 16 | 4 | 15 | 5 | 3 | 3 | 12.5 | 15.3 | 30.9 | 22 |
| AT1G71500 | PSB33 | photosystem b protein 33 | 10 | 6 | 8 | 0 | 0 | 0 | 48.1 | 34.5 | 40.8 | ND |
| AT4G27440 | PORB | protochlorophyllide oxidoreductase B | 9 | 8 | 2 | 8 | 3 | 4 | 32.4 | 39.7 | 15.2 | 24 |
| AT1G03630 | PORC | protochlorophyllide oxidoreductase C | 6 | 6 | 1 | 4 | 0 | 1 | 38.4 | 27.8 | 4 | 17 |
| AT5G42270 | VAR1 | FTSH5 | FtsH extracellular protease family | 12 | 13 | 1 | 12 | 3 | 7 | 26.4 | 31.3 | 8.7 | 21 |
| AT1G50250 | FTSH1 | FTSH protease 1 | 10 | 10 | 1 | 2 | 0 | 0 | 32.8 | 32.8 | 7 | 4 |
| AT2G30950 | VAR2 | FTSH2 | FtsH extracellular protease family | 9 | 9 | 1 | 14 | 6 | 2 | 30.8 | 30.8 | 9.8 | 24 |
| AT1G06430 | FTSH8 | FTSH protease 8 | 6 | 6 | 1 | 0 | 0 | 0 | 15.8 | 15.8 | 7.8 | ND |
| AT5G50920 | CLPC1 | CLPC homolog 1 | 9 | 11 | 6 | 21 | 12 | 8 | 31.5 | 17.7 | 18.7 | 32 |
| AT1G06950 | TIC110 | translocon at the inner envelope membrane of chloroplasts 110 | 19 | 13 | 5 | 22 | 4 | 4 | 33.9 | 23.1 | 15.9 | 29 |
| AT1G44575 | NPQ4, PSBS | chlorophyll A-B binding family protein | 3 | 3 | 3 | 6 | 2 | 3 | 23.8 | 18.9 | 23.8 | 25 |
| AT1G29930 | LHCB1.3 | chlorophyll A/B binding protein 1 | 16 | 15 | 10 | 6 | 4 | 6 | 52.8 | 52.8 | 50.2 | 18 |
| AT3G47470 | LHCA4 | light-harvesting chlorophyll-protein complex I subunit A4 | 6 | 6 | 6 | 5 | 1 | 1 | 4.8 | 39.8 | 39.8 | 32 |
| AT2G05100 | LHCB2.1 | photosystem II light harvesting complex gene 2.1 | 8 | 4 | 8 | 0 | 0 | 0 | 21.5 | 21.5 | 44.2 | ND |
| AT3G27690 | LHCB2.4 | photosystem II light harvesting complex gene 2.3 | 2 | 2 | 2 | 0 | 0 | 0 | 19.2 | 19.2 | 33.8 | ND |
| AT3G08940 | LHCB4.2 | light-harvesting complex photosystem II | 5 | 7 | 4 | 6 | 3 | 5 | 34.8 | 34.8 | 36.9 | 28 |
| AT1G54780 | TLP18.3 | thylakoid lumen 18.3 kDa protein | 2 | 2 | 2 | 3 | 0 | 1 | 37.9 | 28.8 | 37.9 | 14 |
| AT4G01050 | TROL | thylakoid rhodanese-like | 2 | 1 | 1 | 7 | 2 | 2 | 23.2 | 12.9 | 5.6 | 15 |
| AT5G42080 | DL1 | dynamin-like protein | 4 | 4 | 1 | 4 | 2 | 0 | 21.2 | 28.2 | 6.4 | 7.9 |
| AT5G55280 | FTSZ1-1 | homolog of bacterial cytokinesis Z-ring protein FTSZ 1–1 | 2 | 2 | 1 | 2 | 0 | 0 | 20.6 | 20.6 | 5.3 | 9.5 |
| AT3G19820 | DWF1 | cell elongation protein/DWARF1/DIMINUTO (DIM) | 4 | 4 | 1 | 5 | 1 | 0 | 14.6 | 16 | 5.7 | 9.6 |
| AT4G20360 | TRABE1B | RAB GTPase homolog E1B | 35 | 22 | 33 | 23 | 6 | 14 | 60.5 | 56.9 | 60.5 | 59 |
| AT5G60390 | GTP binding Elongation factor Tu family protein | 23 | 18 | 2 | 19 | 3 | 9 | 60.4 | 49 | 14.9 | 41 |
| AT5G13650 | SVR3 | SUPPRESSOR OF VARIEGATION 3 | 2 | 3 | 2 | 2 | 2 | 0 | 18.3 | 17.8 | 10.4 | 4.1 |
| AT5G23060 | CaS | calcium sensing receptor | 15 | 19 | 8 | 16 | 7 | 9 | 52.7 | 52.5 | 34.4 | 35 |
| AT5G66680 | DGL1 | dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48-kDa subunit family protein | 2 | 3 | 1 | 3 | 0 | 0 | 15.8 | 17.6 | 6.2 | 9.6 |
Proteins were considered truly coprecipitated if found in three biological replicates using on-bead digestion or in-gel digestion of protein followed by mass spectrometry. Protein identity was considered if at least two peptide matches were found after MS/MS analysis. AGI, Arabidopsis Genome Initiative; BC, bead control without antibody; DC, dummy antibody control using myc antibody; ND, not determined.