Fig. 1.
Heterophilic interactions between Dsgs and Dscs in SPR. (A) Wild-type Dsg1–Dsg4, Dsc1–Dsc3, or (B) double-mutant Dsg2 W2A A82I and Dsc2 W2A A80I analytes (columns) were tested at concentrations of 3, 6, and 12 µM (12 µM omitted for Dsg4) over surfaces of wild-type Dsg2 (top row), Dsg3, Dsc1, Dsc2, and Dsc3 (bottom row). Responses were normalized for analyte molecular weight differences and are drawn to the same scale across rows. (C) Heterophilic binding affinities (KD) from fitting of SPR data to 1:1 interaction models (SI Appendix, Fig. S1). ±Errors of the fit.