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. 2016 Jun 13;113(26):7243–7248. doi: 10.1073/pnas.1606537113

Fig. 1.

Fig. 1.

Generation of tmc reporter and mutant alleles. (A) Targeting schemes for the generation of the tmcGal4 mutant allele by homologous recombination and the generation of the tmc-Gal4 transgene. Black bars under CG46121-PD indicate the predicted transmembrane segments of Drosophila TMC (33). Red Bars above CG46121-PD indicate the predicted TMC domain (32). (B) Confirmation of the tmcGal4 mutation as a null allele for transcript expression by RT-PCR.