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. 2016 Jun 14;113(26):E3706–E3715. doi: 10.1073/pnas.1607592113

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Fig. 2. — An early block in B-cell development and a gene dose-sensitive MZ B-cell deficiency. (A) Frequencies of major B-cell subsets in bone marrow, peritoneum, and spleen. (B) Expression of the IL-7R α chain in early (B220^loCD43⁺) and late (B220^loCD43⁻) B-cell progenitors. (C) Forward scatter (FSC) profiles of the subsets in B indicate relative sizes of wild-type and Fnip1 mutant cells. (D) Reduced frequency of IgM^hi cells in Fnip1^+/ham heterozygotes (n = 3). (E) Reduced frequency of splenic MZ B cells (CD21/35^hiCD23^lo or CD21/35^hiIgM^hi) and MZ precursors (MZP) (CD21/35^hiIgM^hiCD23^hi). (F) Absolute numbers of transitional (T1, CD93⁺CD23⁻; T2, CD93⁺CD23⁺IgM^int; T3, CD93⁺CD23⁺IgM^hi;), follicular (CD21/35^intCD23^hi), MZP, or MZ B cells in spleen. (G) Serum levels of NP-specific IgM antibodies before (preimmune) and after immunization with NP-Ficoll. Plots in A and E are representative of three mice per genotype. Symbols in B, F, and G represent individual mice, with bars representing the means (± SEM). P values calculated by unpaired two-tailed t test.