Fig. 5.

Elevated AMPK activity in hearts, but not hepatocytes, of Fnip1 mutants. (A) AMPK γ1- and AMPK γ2-specific activity in neonatal heart tissue in the presence or absence of AMP. (B) Total AMPK and AMPK γ2-specific activity in hepatocytes in the presence or absence of metformin. (C) Bone marrow B-cell progenitors (B220⁺CD43⁺) were isolated and treated with rapamycin or vehicle control. Following permeabilization, cells were stained for phosphorylated mTOR (p-mTOR, Ser2448) or phosphorylated S6 ribosomal protein (p-S6, Ser235/236). (A and B) Error bars represent mean ± SEM (n = 4 per genotype). P values calculated by one-way ANOVA with Bonferroni posttest. *Significance at α = 0.05.