| Method |
Lipidic cubic phase |
Lipidic cubic phase |
| Plate type |
96-well plastic sandwich plate |
96-well sitting-drop plate |
| Temperature (K) |
293 |
293 |
| Protein concentration (mg ml−1) |
10 |
10 |
| Buffer composition of protein solution |
20 mM Tris–HCl pH 8.0, 100 mM NaCl, 0.1% DDM |
20 mM Tris–HCl pH 8.0, 0.02% DDM |
| Co-crystallized compound (final concentration in mixture with protein solution) |
0.8 mM macrocyclic peptide |
— |
| Composition of reservoir solution |
30% PEG 300, 100 mM sodium citrate pH 5.0, 100 mM ammonium fluoride |
28–33% PEG 500 DME, 100 mM Tris–HCl pH 7.5–8.0, 50–100 mM magnesium formate |
| Volume of LCP drop (nl) |
50 |
40 |
| Volume of reservoir (nl) |
600 |
800 |
| Soaked compound (final concentration in crystallization drop) |
— |
5 mM Hoechst 33342 |
