Table 1. Antibodies used in the study.
| Primary antibodies | |||||
|---|---|---|---|---|---|
| Target | Antigen | Antibody | Dilution | Source | Cat number |
| Astroglial cells | Glial fibrillary acidic protein (GFAP) |
Rabbit anti-GFAP | 1:1000 | DAKO | Z0334 |
| Brain derived neurotrophic factor receptor |
Tropomyosin receptor kinase B (TrkB) |
Rabbit anti-TrkB | 1:1000 | Cell Signaling |
#4603 Clone 80E3 |
| Gamma- aminobutyric acid (GABA) receptor |
GABA-A receptor alpha1 (GABAARA-1) |
Mouse anti-GABA- AR α-1 IgG1 |
1:10 | NeuroMab | 73-136 Clone N95/35 |
| Glutamate/NMDA receptor |
Glutamate/NMDA receptor epsilon2 (GluN2B/NR2B) |
Mouse anti- GluN2B/NR2B |
1:1000 | NeuroMab | 73-097 Clone N59/20 |
| Housekeeping protein |
β-Actin | Mouse anti-β- actin IgG1 |
1:10000 | Abcam | Ab6276 Clone AC-15 |
| Housekeeping protein |
α-Tubulin | Mouse anti-α- tubulin IgG1 |
1:10000 | Abcam | Ab7291 Clone DM1A |
| Immature neurons | Double cotrin (Dcx) | Goat anti-Dcx | 1:250 | Santa Cruz | SC-8066 |
| Immediate early gene |
c-Fos | Rabbit anti-c-Fos | 1:1000 | EMD | PC38 |
| Mature neurons | Neuronal nuclear protein (NeuN) |
Mouse anti-NeuN | 1:1000 | Millipore | MAB377 |
| Phosphorylated TrkB (Y816) |
pTrkB (Y816) | Rabbit anti-pTrkB (Y816) |
1:1000 | Abcam | Ab75173 |
| Phosphorylated TrkB downstream protein |
Phospho-extracellular- signal-regulated kinase (pErk) |
Rabbit Anti-pErk (Thr202/Tyr204) |
1:1000 | Cell Signaling |
#9101 |
| Phosphorylated TrkB downstream protein |
Phospho-protien kinase B (pAkt) |
Rabbit Anti-pAkt (Ser473) |
1:1000 | Cell Signaling |
#9271 |
| Postsynaptic protein |
Postsynaptic density protein 95 (PSD-95) |
Mouse anti-PSD- 95 IgG2a |
1:100 | NeuroMab | 73-028 Clone K28/43 |
| Presynaptic protein | Synaptophysin | Mouse anti- synaptophysin |
1:2000 | Alomone labs |
ANR-013 |
| Replicating cells | Bromodeoxyuridine (BrdU) |
Rat anti-BrdU | 1:1000 | Abcam | Ab6326 |
| TrkB downstream protein |
Extracellular-signal- regulated kinase (Erk) |
Rabbit Anti-Erk | 1:1000 | Assay designs |
KAP-MA001 |
| TrkB downstream protein |
Protien kinase B (Akt) | Mouse Anti-Akt | 1:1000 | Cell Signaling |
#2920S |
| Secondary antibodies | |||||
|---|---|---|---|---|---|
| Label | Host | Target | Dilution | Source | Cat Number |
| Alexa 488 | Goat | Mouse IgG | 1:1000 | Invitrogen | A11001 |
| Alexa 647 | Goat | Rabbit IgG | 1:1000 | Invitrogen | A21244 |
| Alexa 647 | Donkey | Goat IgG | 1:1000 | Invitrogen | A10522 |
| Biotin | Rabbit | Goat IgG | 1:1000 | Vector | BA5000 |
| Biotin | Goat | Rabbit IgG | 1:1000 | Vector | BA1000 |
| Cy 3 | Goat | Rat IgG | 1:1000 | Invitrogen | A21447 |
| IR Dye 680 | Goat | Mouse IgG | 1:20000 | Li-Cor | 926-32210 |
| IR Dye 800 | Goat | Mouse IgG | 1:20000 | Li-Cor | 926-32220 |
| IR Dye 680 | Goat | Rabbit IgG | 1:20000 | Li-Cor | 926-32221 |
| IR Dye 800 | Goat | Rabbit IgG | 1:20000 | Li-Cor | 926-32211 |