Table 3.

Protein content measured by Western blotting

	Young men		Older men
Protein	Control leg	Immobilized leg	Control leg	Immobilized leg	Main effect
Lipoprotein lipase	0.95 ± 0.18	1.07 ± 0.21	1.30 ± 0.19	1.53 ± 0.22	(*)
Hormone‐sensitive lipase	0.60 ± 0.10	0.48 ± 0.07	0.53 ± 0.06	0.64 ± 0.14
pAMPKthr172	1.08 ± 0.09	1.15 ± 0.09	1.00 ± 0.12	1.12 ± 0.13
AMPKα1	0.95 ± 0.09	1.03 ± 0.06	0.91 ± 0.11	1.02 ± 0.10	†
AMPKα2	0.97 ± 0.03	0.95 ± 0.02	0.93 ± 0.03	0.96 ± 0.04
ΑΜΡΚβ1	0.90 ± 0.09	1.14 ± 0.09	0.97 ± 0.12	1.24 ± 0.11	†
ΑΜΡΚβ2	1.24 ± 0.08*, †	1.11 ± 0.05	0.98 ± 0.06	0.99 ± 0.05
ΑΜΡΚγ1	1.35 ± 0.07	1.44 ± 0.09	1.21 ± 0.11	1.34 ± 0.13
ΑΜΡΚγ3	1.90 ± 0.17	1.64 ± 0.16	1.37 ± 0.13	1.21 ± 0.10	†

Data are mean values ± SEM. *P < 0.05 and (*)0.1 > P < 0.05 young vs. older men; ^† P < 0.05 immobilized leg vs. control leg. It is a main effect of either group or leg if marked in the ‘main effect’ column or an interaction between group and leg if marked in the table (AMPKβ2). See representative Western blots in Fig. 7.