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. 2016 May 3;291(27):13905–13916. doi: 10.1074/jbc.M116.731281

FIGURE 1.

FIGURE 1.

Ctr1 ectodomain cleavage occurs in a Ctr2-stimulated, cysteine protease-dependent manner. A, model showing monomeric full-length Ctr1 and tCtr1, with Met and His residues in the ectodomain indicated in blue and red, respectively, and glycans indicated with branches. B, cells containing a doxycycline-inducible Ctr2 (Tet-On Ctr2) were treated with 100 ng/ml doxycycline for the times indicated, harvested, and immunoblotted with anti-Ctr2, anti-Ctr1 (T, truncated; F, full-length), and anti-tubulin antibody. C, MEFs were treated with DMSO, 2 μg/ml TIMP-2, TIMP-3, and TAPI-2 or 5 μm BB-94 for 16 h, and protein extract was analyzed by immunoblotting with anti-Ctr1 (T, truncated; F, full-length) and anti-tubulin antibody. D, MEFs were treated with 10 μm E64d, and CHO and HEK cells were treated with 50 μm E64d for 16 h before immunoblot analysis as in B. E, Tet-On Ctr2 cells were cultured with or without 100 ng/ml doxycycline for 24 h before treatment with either DMSO or E64d (50 μm), and protein extracts were analyzed with immunoblotting as in B.