. 2016 May 9;291(27):13955–13963. doi: 10.1074/jbc.M116.723676

TABLE 3.

Intrinsic fluorescence parameter FIR

The mean (n = 3) and the standard deviation are represented. The FIR parameter is calculated from the intensities at 320 and 360 nm for all the proteins.

	FIR ± S.D.
PimA WT
In solution	1.30 ± 0.01
DOPC	1.35 ± 0.01
DOPC/DOPG	1.25 ± 0.04
DOPC/PI	1.43 ± 0.01

PimA Trp-82
In solution	1.03 ± 0.01^a
DOPC	1.04 ± 0.03^a
DOPC/DOPG	1.18 ± 0.01^a
DOPC/PI	1.29 ± 0.04^a

PimA Trp-154
In solution	1.15 ± 0.05
DOPC	1.23 ± 0.04
DOPC/DOPG	1.31 ± 0.01
DOPC/PI	1.35 ± 0.03

PimA Trp-349
In solution	1.70 ± 0.01
DOPC	1.74 ± 0.004
DOPC/DOPG	1.66 ± 0.005
DOPC/PI	1.84 ± 0.02

^a Noteworthy, the FIR parameter for PimA Trp-82 is calculated using the intensities at 310 and 340 nm due to a large change in the shape of the spectra as a consequence of the strong shift in the maximum of emission.