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. 2016 May 9;291(27):13955–13963. doi: 10.1074/jbc.M116.723676

TABLE 4.

Tryptophan anisotropy changes upon SUV interaction

The mean (n = 303) and standard deviations are represented. The t test showed no statistical significance between Trp-82 in solution versus the presence of DOPC SUVs (p = 0.344). Likewise, PimA Trp-154 t test showed no statistical significance between Trp-154 in solution versus the presence of DOPC SUVs (p = 0.3051). Nevertheless, PimA Trp-349 values revealed significant differences between in solution versus DOPC and/or DOPC/DOPG. However, the values obtained in the presence of PI-containing liposomes were not statistically significant (n = 303; p = 0.0589).

r ± S.D.
PimA-Trp-2
    In solution 0.12 ± 0.01
    DOPC 0.12 ± 0.01
    DOPC/DOPG 0.12 ± 0.01
    DOPC/PI 0.14 ± 0.04

PimA-Trp-54
    In solution 0.10 ± 0.01
    DOPC 0.10 ± 0.01
    DOPC/DOPG 0.12 ± 0.03
    DOPC/PI 0.12 ± 0.01

PimA-Trp-349
    In solution 0.13 ± 0.02
    DOPC 0.12 ± 0.02
    DOPC/DOPG 0.11 ± 0.02
    DOPC/PI 0.13 ± 0.04