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. 2016 Apr 29;291(27):14023–14033. doi: 10.1074/jbc.M116.726687

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Overexpression of CaHI drastically changed (Z)-3-hexenal and (E)-2-hexenal composition in transgenic tomatoes. A, (Z)-3- and (E)-2-hexenal analysis in wild-type and CaHI-overexpressing tomato (oxCaHI) leaves. Volatiles were collected by SPME and then analyzed by GC-MS. SIM (Selected ion monitoring, m/z = 98) chromatograms to detect (Z)-3- and (E)-2-hexenals are shown. Inset, confirmation of enhanced expression of CaHI in transgenic tomatoes by qRT-PCR (the actin gene SlACT was used as an internal standard). Numbers indicate transgenic tomato lines showing high CaHI expression. B, SIM chromatogram (m/z = 98) of volatiles from ripe tomato fruits.