TABLE 1.
Summary of purification of CaHI from red paprika
Total protein | Total activity | Specific activity | Purification | Yield | |
---|---|---|---|---|---|
mg | nmol/min | nmol/min mg | -Fold | % | |
Crude extracta | 16,800 | 647,000 | 38.3 | 1.0 | 100 |
Phenyl-Sepharose | 866 | 130,000 | 150 | 3.92 | 20.1 |
Hydroxyapatite | 153 | 98,700 | 646 | 16.9 | 15.3 |
Mono Q5/5 | 0.80b | 10,000 | 12,600 | 328 | 1.56 |
Mono Q1.6/5 | 0.012b | 3,840 | 320,000 | 8,350 | 0.59 |
a Prepared from 640 g of red paprika pericarp.
b Protein concentration was estimated using EA 280 (0.1% protein) = 1.