Skip to main content
. 2016 Apr 29;291(27):14023–14033. doi: 10.1074/jbc.M116.726687

TABLE 1.

Summary of purification of CaHI from red paprika

Total protein Total activity Specific activity Purification Yield
mg nmol/min nmol/min mg -Fold %
Crude extracta 16,800 647,000 38.3 1.0 100
Phenyl-Sepharose 866 130,000 150 3.92 20.1
Hydroxyapatite 153 98,700 646 16.9 15.3
Mono Q5/5 0.80b 10,000 12,600 328 1.56
Mono Q1.6/5 0.012b 3,840 320,000 8,350 0.59

a Prepared from 640 g of red paprika pericarp.

b Protein concentration was estimated using EA 280 (0.1% protein) = 1.