TABLE 2.

Kinetic parameters of purified CaHI and recombinant HIs

Ca-, C. annuum; Sl-, S. lycopersicum; St-, S. tuberosum; Ms-, M. sativa; Cs-, C. sativus; Os-, O. sativa; r-, recombinant.

	kcat	Km	kcat/Km
	s−1	mm	s−1 mm−1
CaHI	760	0.73	1040
rCaHI	521 ± 13.0	1.78 ± 0.06	293 ± 2.60
rSlHI1	264 ± 18.1	0.20 ± 0.04	1349 ± 126
rStHI1	27.7 ± 1.75	0.33 ± 0.03	83.7 ± 1.08
rStHI2	159 ± 13.9	0.72 ± 0.10	224 ± 13.7
rMsHI	308 ± 13.9	0.32 ± 0.04	990 ± 79.1
rCsHI1	951 ± 66.2	0.56 ± 0.08	1749 ± 172
rOsHI1	43.5 ± 2.23	1.23 ± 0.12	35.8 ± 1.61