FIGURE 4.

Identification of the putative Nur77 binding site on the surface of the anti-apoptotic Bfl-1 protein. A–C, water-accessible surface representation of Bfl-1 colored by electrostatic potential (± 5 kT/e⁻) showing the Nur77 binding site within the area enclosed by a circle (A), after a 90° rotation over the x axis to show a very distinct BH3 binding site (B), and after a 90° rotation over the y axis (C). The Nur77 peptide, presumed to be partially helical (depicted as a red cylindrical object), and the BH3 peptide ligand (depicted as a blue cylindrical object) bind at distant sites separated by a common wall formed by helices α2 and α3 (dashed line).