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Kinetic parameters of SdeAgu115A on selected substrates: XU4m2XX (A), U4m2XX (B), beechwood xylan (C), and pNP-GlcAp (D). Data corresponding to A, B, and D were fitted to the Michaelis-Menten equation. Activity data collected using beechwood xylan were fitted to a conventional substrate inhibition equation. The normalized concentration of the MeGlcAp in the glucuronoxylan (mm) is presented as km(app). Error bars, S.D.
