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. 2016 May 11;291(27):14160–14169. doi: 10.1074/jbc.M116.728980

FIGURE 4.

FIGURE 4.

SDS-PAGE analysis and catalytic activity of a series of mutant semisynthetic PTENs toward a range of diC6-PIP3 substrate concentrations. A, Coomassie-stained 10% SDS-PAGE gel of semisynthetic PTEN mutants. First lane, molecular mass markers; second lane, BB-4p-PTEN; third lane, Cα2A-4p-PTEN; fourth lane, Cα2A-t-PTEN; fifth lane, Cα2D-4p-PTEN; sixth lane, Cα2D-t-PTEN; seventh lane, 4p-ePTEN; eighth lane, 3R/D-4p-PTEN; ninth lane, 3R/D-t-PTEN. B, catalytic activity of ePTEN forms with diC6-PIP3 (n = 2): Q17R, R41G, E73D, N262Y, and N329H. C, catalytic activity of Cα2 loop mutant PTEN forms with diC6-PIP3. Cα2D, K327D, K330D, K332D, and R335D; Cα2A, K327A, K330A, K332A, and R335A (n = 2).