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. 2016 May 11;291(27):14160–14169. doi: 10.1074/jbc.M116.728980

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Alkaline phosphatase sensitivity of different semisynthetic phospho-PTEN mutants. A, Western blot of time course from the alkaline phosphatase-treated 4p-ePTEN (Q17R, R41G, E73D, N262Y, and N329H) and WT 4p-PTEN. B, quantification of the alkaline phosphatase assay for 4p-ePTEN and WT 4pPTEN (n = 3). C, Western blot of time course from the alkaline phosphatase treated Cα2D-4p-PTEN (K327D, K330D, K332D, and R335D), Cα2A-4p-PTEN (K327A, K330A, K332A, R335A), and WT 4p-PTEN. D, quantification of the alkaline phosphatase assay for 4p-Cα2 loop mutants and WT 4p-PTEN (n = 6). Error bars indicate ± standard error.