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Mass spectroscopic analysis of Ste24p cleavage products. A and B, mass spectra of the M16A reporter in the absence (A) and presence of purified SmSte24p (B). C, the peaks identified in A and B correspond to the indicated species. Peptide 3 is a minor contaminating peptide that lacks the Asn residue present in peptide 1. D, analysis of mass spectra (see Figs. 10 and 11) reveals cleavage sites in the indicated reporter substrates. Triangles, major cleavage sites that were identified.
