TABLE 1.
Kinetics of Ste24p cleavage of K-Ras4b reporter in the presence of increasing concentrations of insulin B-chain
All reaction conditions were the same as described in the legend to Fig. 5A. Data shown represent one of two biological replicates. Similar values and trends for Km and Vmax parameters were observed in both experiments. S.E. values shown represent error with respect to curve fitting using Prism version 6.
| Insulin B-chain |
||||
|---|---|---|---|---|
| 0 μm | 10 μm | 20 μm | 33 μm | |
| Km (μm) | 23.2 ± 0.8 | 29.7 ± 1.2 | 37.5 ± 2.8 | 37.5 ± 2.3 |
| Vmax (μm/min) | 0.56 ± 0.01 | 0.52 ± 0.02 | 0.56 ± 0.03 | 0.46 ± 0.02 |
| Hill coefficient | 2.04 ± 0.13 | 2.49 ± 0.20 | 2.16 ± 0.21 | 2.38 ± 0.23 |