In vitro differentiation potential of iNSCs.
A, C, and E, differentiation potential of e-iNSCs and r-iNSCs into astrocytes (A), neurons (C), and oligodendrocytes (E), as shown by immunostaining with antibodies against GFAP, Tuj1, and MBP, respectively. B, D, and F, the efficiency of differentiation into astrocytes (B), neurons (D), and oligodendrocytes (E) was compared by quantifying the numbers of GFAP, Tuj1, and MBP positive cells, respectively. Control NSCs were used as a positive control for determining the differentiation efficiency. Scale bars, 100 μm. ***, p < 0.001.