TABLE 2.

Primers used for DNA methylation analysis

Gene name	Sequence	Annealing temperature
Nestin enhancer 1st	5′-GATCCCAGTGTGGTGGTACG-3′	45 °C
	5′-GGCTTCAGCTCCGTCTCCAT-3′
Nestin enhancer 2nd	5′-GTGTGGTGGTACGGGAAATC-3′	60 °C
	5′-GAGAAGGACGGGAGCAGAG-3′
Col1a11st	5′-GTTAGGTAGTTTTGATTGGTTGG-3′	55 °C
	5′-ACAATAACCCCTAAAAAAAACAAAAA-3′
Col1a12nd	5′-TGGTATAAAAGGGGTTTAGGTTAGT-3′	60 °C
	5′-ACAATAACCCCTAAAAAAAACAAAAA-3′