FIGURE 1.

Identification of qrr transcriptional start sites in V. vulnificus. A, qrr transcriptional start sites were identified through primer extension experiments. T, C, G, and A represent the sequencing ladders. Transcriptional start sites are indicated with arrows on the gel images, and are boxed in the sequence to the right of each image. B, alignment of the qrr coding regions and upstream regions was performed using ClustalW. The transcriptional start sites are denoted with arrows, and the putative σ⁵⁴-binding sites and Rho-independent terminator sequences are underlined. Putative LuxO-binding sites perfectly matched to the consensus sequences are denoted with thick-line boxes, and those partially matched with the consensus sequence are denoted with thin-line boxes.