FIGURE 6.

Twinkle helicase dissociates mobile D-loop DNA structures. A and B, indicated Twinkle helicase hexamer concentrations were incubated with the specified mobile D-loop DNA substrate (0.5 nm) at 37 °C for 30 min as described under “Experimental Procedures.” Reaction products were resolved on non-denaturing 12% polyacrylamide gels. Representative gel images from at least three independent experiments are shown. C, quantitative analysis of percent D-loop dissociation in the 5′ to 3′ direction (substrate 14) or 3′ to 5′ direction (substrate 15) (A, filled circle (substrate 14); B, open circle (substrate 15)). Average values of at least three independent experiments with standard deviations indicated by error bars are shown.