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. 2016 May 11;291(27):14324–14339. doi: 10.1074/jbc.M115.712026

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Twinkle tolerates a single cyclopurine in the helicase translocating or non-translocating strands. A and C, indicated Twinkle helicase hexamer concentrations were incubated with the specified cyclo-dA (A) containing DNA substrates (substrates 19, 20) or cyclo-dG (C) containing DNA substrates (substrates 21 and 22) (0.5 nm) at 37 °C for 30 min as described under “Experimental Procedures.” Reaction products were resolved on non-denaturing 12% polyacrylamide gels. Representative gel images from at least three independent experiments are shown. B and D, quantitative analysis of percent DNA substrate unwound from A and C, respectively. Average values of at least three independent experiments with standard deviations indicated by error bars are shown. Undamaged substrate (substrate 16), filled circle; bottom (non-translocating) strand cPu substrate (substrates 19, 21), open circle; top (translocating) strand cPu substrate (substrates 20, 22), filled triangle.