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IFNγ down-regulates CD31 and reduces NLC-mediated survival of CLL cells. A, NLCs (n = 13 donors) were treated without or with 10 ng/ml IFNγ for 72 h, and CD31 was measured using qPCR. UT, untreated. B, NLCs were treated as above and then co-cultured with non-autologous CLL cells for 24 h. Annexin V FITC-propidium iodide staining was done with CLL cells, and double-negative cells were counted as live (n = 4 donors).
