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. Author manuscript; available in PMC: 2016 Jul 5.
Published in final edited form as: Nat Methods. 2014 Dec 22;12(2):140–146. doi: 10.1038/nmeth.3217

Figure 3. Simultaneous fast calcium imaging and photostimulation of multiple neurons in vivo.

Figure 3

(a) A field of view of neurons in layer 2/3 of somatosensory cortex colabeled with C1V1-2A-mCherry and GCaMP6s (scale bar, 100 μm). Ten neurons were targeted for simultaneous photostimulation (white circles).

(b) Calcium transients from the ten photostimulated neurons showing the responses on all individual trials.

(c) Color-coded plot of the strength of photostimulation as measured by the sum of inferred spikes immediately post-stimulation. Stimulated neurons are circled in black. 300 total neurons, 120 trials, mean inferred action potentials post photostimulation = 1.0 ± 0.1 (mean ± SD, black arrow in legend).