Typical loading behaviour of CT-based systems studied via UV-vis incubation experiments with dyes of varied electrostatic charge. Absorbance curve of MB-containing solution during incubation with hydrogels CT(2%)-PhS (a). Peak of MB absorbance at 661 nm is decreased at increased incubation time points (0, 12, 24, 36, 48, 60, 84 h), providing evidence of MB loading in the CT hydrogel. MB (b), MO (c) and AR (d) loading curves in hydrogels CT(2%)-PhS (–■–), CT(2%)-4Ph (--♦--), CT(2%)-PEG (··▲··).Triplicates were applied for the loading of hydrogels CT(2%)-PhS with MO; minimal standard deviation was observed between replicas at each loading time point, giving supporting evidence of the system reproducibility. Hydrogels are selectively loaded with dyes of opposite electrostatic charge, due to the electrostatic hydrogel-dye complexation occurring following hydrogel incubation.