TABLE 1.
Specifications of the non-isoform-specific TaqMan assays (Life Technologies, Inc.) used for RT-PCR validation
| Protein | Gene | Assay ID |
|---|---|---|
| β-Glucuronidase | GUSB | Hs00939627a |
| Elongin C | TCEB1 | Hs00855349 |
| Prolyl hydroxylase domain-containing protein 2 (PHD2) | EGLN1 | Hs00254392 |
| 6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 | PFKFB3 | Hs00998700 |
| Triose-phosphate isomerase | TPI1 | Hs03806547 |
| Phosphoglycerate kinase 1 | PGK1 | Hs00943178 |
| Pyruvate kinase muscle | PKM | Hs00761782 |
| Pyruvate dehydrogenase kinase isozyme 1 | PDK1b | Hs01561850 |
| Transketolase-like 1 | TKTL1 | Hs00202061 |
a This is an endogenous control gene.
b This should not be confused with phosphoinositide-dependent kinase 1 protein.