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HPLC separation of lipid extracts from fad3-2 fad7-2 fad8 plants exposed to 14C-MDA for 24 h. A, polar leaf lipids from 14 C-MDA treated fad3-2 fad7-2 fad8 (fadt) plants, separated by HPLC and monitored by light absorption. B, radiation distribution in HPLC-fractions measured by liquid scintillation. (Pool of leaves from 5 plants, 400,000 dpm/plant l−1; SA: 0.9 nCi nmol−1).
