TABLE 1.
Antibodies used in the study
| Antibody | Specificity | Host species | Dilution | Source or Ref. |
|---|---|---|---|---|
| 17025 | Raised against human recombinant tau | Rabbit | 1:1000 (WB)a | 52 |
| PHF-1 | p-Tau (phosphorylated at Ser-396 and Ser-404) | Mouse | 1:1000 (WB), 1:2000 (ICC) | Gift from Dr. Peter Davies |
| AT8 | p-Tau (phosphorylated at Ser-202 and Thr-205) | Mouse | 1:500 (ICC) | Innogenetics |
| GAPDH (6C5) | Glyceraldehyde-3-phosphate dehydrogenase | Mouse | 1:3000 (WB) | Advanced Immunochemical |
| PM036 | LC3 | Rabbit | 1:500 (WB) 1:2000 (ICC) | MBL |
| 2C11 | p62 | Mouse | 1:1000 (ICC) | Abnova |
| 610832 | P62 | Mouse | 1:1000 (WB) | BD Transduction |
| H4A3 | LAMP1 | Mouse | 1:1000 (ICC) | BD Biosciences |
| Ub1b4 | Ubiquitin | Mouse | 1:1000 (WB), 1:1000 (ICC) | 53 |
| PW8155 | 20S proteasome | Rabbit | 1:2000 (ICC) | Biomol |
| 81A | p-α-Syn (phosphorylated at Ser-129) | Mouse | 1:5000 (ICC) | 54 |
| MJF-R13 | p-α-Syn (phosphorylated at Ser-129) | Rabbit | 1:2000 (ICC) | Michael J. Fox Foundation |
a WB, Western blotting; ICC, immunocytochemistry.