FIGURE 5.

Mus101 inhibits Top2-mediated kinetoplast DNA decatenation. A, 0.5 μg of untreated or λ-phosphatase (λ-PPase)-treated Top2 was incubated with 0.1 μg of kinetoplast DNA in the presence of Mus101[1–350]-His₆ (at 0, 0625, 1.25, or 2.5 μm) at 30 °C for 15 min. The samples were analyzed by electrophoresis in 1% agarose gel with EtBr. B, triplicate results were quantified, and relative ratios of DNA products were plotted against Mus101[1–350]-His₆ concentrations. Top2 (untreated) decatenation activity reduces significantly with the increasing amount of Mus101.