FIGURE 4.
Biochemical property of HcaR. A, size exclusion chromatography of AcHcaR protein. The absorbance at 280 nm is plotted in absorbance units versus retention volume in milliliters for AcHcaR. The inset is the plot of Kav coefficient versus logarithm of molecular weight. Red circles correspond to standard proteins: 1, aprotinin (6.5 kDa); 2, ribonuclease A (13.7 kDa); 3, carbonic anhydrase (29 kDa); 4, ovalbumin (43 kDa); 5, conalbumin (75 kDa); 6, aldolase (158 kDa); and 7, thyroglobulin (669 kDa). The blue circle is AcHcaR (corresponding to 95 kDa). A single peak corresponding to a tetramer is observed. B, the structure of the AcHcaR tetramer as predicted by the PISA server. C, a similar AcHcaR tetramer is maintained in the AcHcaR·DNA complex.