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. 2016 May 2;291(25):13257–13270. doi: 10.1074/jbc.M116.718189

FIGURE 5.

FIGURE 5.

Pharmacological inhibition of ezrin by NSC305787 and NSC668394 up-regulates the expression of integrated stress response signature genes in a dose-dependent manner. A, human MG63.3 and mouse K7M2 osteosarcoma cells were treated with 20.0 μmol/liter MMV667492 or vehicle control for 6 h, and mRNA levels of PTGS2, ATF3, DDIT3, DDIT4, TRIB3, and ATF4 were measured by real time qPCR. The results were normalized to 18S rRNA levels and expressed as fold change over the control group. The values are presented as the means and standard deviations of triplicate determinations (*, p < 0.05, compared with control; using an unpaired Student's t test). B, K7M2 mouse osteosarcoma cells were treated with indicated concentrations of compounds, NSC305787 or NSC668394, or vehicle control for 6 h, and mRNA levels of PTGS2, ATF3, DDIT3, DDIT4, TRIB3, and ATF4 were measured by real time qPCR. The results were normalized to 18S rRNA levels and expressed as fold change over the control group. The values are presented as the means and standard deviations of triplicate determinations (*, p < 0.05, compared with control; using an unpaired Student's t test; ns, non-significant).