TABLE 3.
Estimated MIC values for inhibition of galectin-3-induced hemagglutination in V-shaped plates and gel cards
As the MIC in this assay is somewhere between the concentration not able to inhibit the hemagglutination and the one able to do so, we define here the MIC values as concentration intervals (lowest concentration indicates no inhibition observed; highest concentration indicates no hemagglutination observed). NI indicates no inhibition at highest concentration tested. Blank field indicates not tested. NA means not applicable.
Samples | Highest [sample] tested | V-plate [galectin-3] (μm) |
Highest [sample] tested | Gel card [galectin-3] (μm) |
||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 5 | 10 | 5 | 10 | |||
PectaSol-C® (mg/ml) | 5 mg/ml | NI | 6.7 mg/ml | NI | ||||
FPP (mg/ml) | 5 mg/ml | NI | NA | |||||
MCP (mg/ml) | 5 mg/ml | NI | NI | 2.7 mg/ml | NI | |||
RG-I-4 (mg/ml) | 5 mg/ml | NI | 5a | NA | ||||
p-oligo (mg/ml) | 1 mg/ml | NI | NA | |||||
p-oligo H (mg/ml) | 1 mg/ml | NI | 3.3 mg/ml | NI | ||||
p-oligo J (mg/ml) | NA | 3.3 mg/ml | NI | |||||
p-oligo L (mg/ml) | NA | 3.3 mg/ml | NI | |||||
Davanat® (mg/ml) | 5 mg/ml | NI | 13.3 mg/ml | NI | ||||
β-Lactose (μm) | 2000 μm | 12.5–25 | 250–500 | 1000–2000 | 1500a | 1500 μm | 375–750 | 300–1500 |
33DFTG (μm) | 20 μm | 0.25–0.74 | 2.22–6.67 | 20 μm | 2.2–6.67 |
a Partial inhibition at the highest concentration was tested.