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. 2016 Apr 26;291(25):13318–13334. doi: 10.1074/jbc.M116.721464

TABLE 3.

Estimated MIC values for inhibition of galectin-3-induced hemagglutination in V-shaped plates and gel cards

As the MIC in this assay is somewhere between the concentration not able to inhibit the hemagglutination and the one able to do so, we define here the MIC values as concentration intervals (lowest concentration indicates no inhibition observed; highest concentration indicates no hemagglutination observed). NI indicates no inhibition at highest concentration tested. Blank field indicates not tested. NA means not applicable.

Samples Highest [sample] tested V-plate [galectin-3] (μm)
Highest [sample] tested Gel card [galectin-3] (μm)
1 2 5 10 5 10
PectaSol-C® (mg/ml) 5 mg/ml NI 6.7 mg/ml NI
FPP (mg/ml) 5 mg/ml NI NA
MCP (mg/ml) 5 mg/ml NI NI 2.7 mg/ml NI
RG-I-4 (mg/ml) 5 mg/ml NI 5a NA
p-oligo (mg/ml) 1 mg/ml NI NA
p-oligo H (mg/ml) 1 mg/ml NI 3.3 mg/ml NI
p-oligo J (mg/ml) NA 3.3 mg/ml NI
p-oligo L (mg/ml) NA 3.3 mg/ml NI
Davanat® (mg/ml) 5 mg/ml NI 13.3 mg/ml NI
β-Lactose (μm) 2000 μm 12.5–25 250–500 1000–2000 1500a 1500 μm 375–750 300–1500
33DFTG (μm) 20 μm 0.25–0.74 2.22–6.67 20 μm 2.2–6.67

a Partial inhibition at the highest concentration was tested.