TABLE 1.

Nucleotide sequences of the synthetic primers used in the PCR amplifications to generate the deleted and mutant variants of PHOX2B

The enzyme restriction sites used for cloning are underlined.

Construct name	Forward primer (5′–3′)	Reverse primer (5′-3′)
PHOX2B Nter	CGGAATTCCAATGTATAAAATGGAATATTC	GCGAATTCTCACTTGCGCTTCTCGTTG
PHOX2B Nter + HD	CGGAATTCCAATGTATAAAATGGAATATTC	GCGAATTCTCACTCCTGCTTGCGAAAC
PHOX2B HD	CGGAATTCAGCAGCGGCGCATCC	GCGAATTCTCACTCCTGCTTGCGAAAC
PHOX2B HD + Cter	CGGAATTCAGCAGCGGCGCATCC	ATGAATTCGGCTTCCGCCGCAGG
PHOX2B Cter	CGGAATTCTTCGCAAGCAGGAGCGC	ATGAATTCGGCTTCCGCCGCAGG
PHOX2B ΔNLS1	CTCAACGAGACCACTTTCACCAGTGCCCAG	AAAGTGGTCTCGTTGAGGCCGCCGTG
PHOX2B ΔNLS2	GTTCCAGCAGGAGCGCGCAGCG	TCCTGCTGGAACCACACCTGGACTCGC
PHOX2B Δ106–147	ACCACTTTCAACCGCCGCGCCAAG	CGGTTGAAAGTGGTGCGGATGCG
External primers (for ΔNLS1, ΔNLS2, Δ NLS1-2 and Δ106–147)	CACAAGCTTGCTGCGGAATTGTACC	CTCCATTCGCCCCGCAGCTG
GAL4 BD-/ VP16 AD-PHOX2B WT	GGGGTACCATGTATAAAATGGAATATTC	CCGGTACCGGCTTCCGCCGCAGG
GAL4 BD-/ VP16 AD-Nter	GGGGTACCATGTATAAAATGGAATATTC	CCGGTACCTCACTTGCGCTTCTCGTTG
GAL4 BD-/ VP16 AD-Nter + HD	GGGGTACCATGTATAAAATGGAATATTC	CCGGTACCTCACTTGCGCTTCTCGTTG
GAL4 BD-/ VP16 AD-HD	GGGGTACCCAGCGGCGCATCC	CCGGTACCTCACTTGCGCTTCTCGTTG
GAL4 BD-/ VP16 AD-HD + Cter	GGGGTACCCAGCGGCGCATCC	CCGGTACCGGCTTCCGCCGCAGG
GAL4 BD-/ VP16 AD-Cter	GGGGTACCCGCAAGCAGGAGCGC	CCGGTACCGGCTTCCGCCGCAGG
GAL4 BD-/ VP16 AD-PHOX2A	GCGGCCGCCGGGCCGATGGACTACTCCTACC	TTGCGGCCGCCTAGAAGAGATTGGTCTTCAGGGC