TABLE 2.

Effects of adding amino acids to MtbAldR and MtbAldR-DNA complex, respectively

Amino acids	ANS intensity change upon adding respective amino acids to MtbAldR (7 μm)	Effect on the MtbAldR (650 nm)-DNA complex	Changes to the quaternary structure of MtbAldR (0.5 mg/ml)a
Tyrosine	Increase	Inhibition	—b
Phenylalanine	Increase	No effect	Hexamer to heptamer
Tryptophan	Increase	Inhibition	—b
Histidine	Increase	No effect
Aspartate	Increase	Inhibition	Hexamer to heptamer
Leucine	Increase	No effect	No effect
Glutamate	Increase	Partial inhibition	Hexamer to decamer
Asparagine	Decrease	Partial inhibition	Hexamer to tetradecamer
Glutamine	Decrease	No effect	Hexamer to dodecamer
Alanine	Decrease	Inhibition	—a

^a Smaller changes to the oligomeric state of MtbAldR in the presence of amino acids like alanine can be interpreted as a transition to an “open” quaternary association, as hypothesized under “Discussion.”

^b Inconclusive results; experiment was not performed.