FIGURE 8.

Compounds counteract Aβ associated net spine loss in rat primary hippocampal neurons. A, representative spine segments (23 microns) of primary neurons dosed with Aβ, or Aβ plus BTA-EG₆, or BAMs 1–3 compared with control (0.1% DMSO) and quantitative representation of spine number per micron for all dosing experiments compared with control. B, representative spine segments (23 microns) of primary neurons pre-treated with 5 μm BTA-EG₆ or BAMs 1–3 for 24 h followed by addition of aggregated Aβ. C, relative expression levels of p-Cofilin/total Cofilin in primary neurons treated with Aβ alone or Aβ plus BTA-EG₆ or BAMs 1–3 compared with control expression levels. Data are expressed as mean values ± S.E., n = 42. ##, p ≤ 0.01, as determined by unpaired t test compared with control. *, p ≤ 0.001; *, p ≤ 0.0001 as determined by unpaired t test compared with cells treated with Aβ alone.