Skip to main content
. 2016 Apr 8;291(23):12029–12038. doi: 10.1074/jbc.M116.728170

FIGURE 2.

FIGURE 2.

Sgpp2 mRNA is differentially expressed in tissues. A, Sgpp2−/− mice at 4 days after birth showed normal skin appearance and morphology (middle mouse) similar to WT mice (mouse on the left). In contrast, Sgpp1−/− mice (mouse on the right) displayed severe ichthyosis. B, mRNA expression for Sgpp2 (top gel) and Gapdh (bottom gel) determined by semiquantitative real-time PCR of various tissues from Sgpp2+/+ (WT) and Sgpp2−/− (KO) mice. C and D, relative mRNA expression, normalized to Gapdh mRNA expression, for Sgpp2 (C) and Sgpp1 (D) was determined by quantitative real-time PCR of various tissues from Sgpp2+/+ (WT) and Sgpp2−/− (KO) mice. Bars, mean ± S.D. (error bars); Student's t test, n = 4 for each genotype. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. E, Sgpp2 mRNA expression was determined by quantitative real-time PCR in various tissues from WT mice. Values are shown as the relative level of Sgpp2 mRNA normalized to Gapdh mRNA expression with brain Sgpp2 set to 1. Bars, mean ± S.D.; n = 3.