FIGURE 9.

UBD KD increases cytokine-induced apoptosis in human islets and human EndoC-βH1 cells. Human islet cells (A and B) and the human beta cell line EndoC-βH1 (C and D) were transfected with siC and two different siUBDs (si-hU1 and si-hU2). 48 h later, cells were left untreated or treated with IL-1β + IFN-γ (IL+IFN) for 24 h. UBD KD was evaluated by RT-PCR and normalized by the housekeeping gene β-actin (A and C), and apoptosis was analyzed by propidium iodide/Hoechst staining (B and D). Results are represented as a box plot indicating lower quartile, median, and higher quartile, with whiskers representing the range of the remaining data points in B–D and as box plot indicating the single experiments, the average, and the S.E. in A. *, p < 0.05, and ***, p < 0.001 versus siC untreated; #, p < 0.05; ##, p < 0.01; and ###, p < 0.001 as indicated by bars; one-way ANOVA followed by Student's paired t test with Bonferroni correction. n = 3–7 independent experiments.