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. 2016 Apr 4;291(23):12040–12056. doi: 10.1074/jbc.M115.704619

TABLE 3.

Plasmids used in the study

Plasmids Encoding protein
pSEL-IRE1α bait Cytosolic domain of IRE1α
pSEL-IRE1K599A bait Cytosolic domain of IRE1α with K599A mutation
pSEL-IRE1Kin bait Kinase domain of IRE1α
pSEL-IRE1Ken bait Endonuclease domain of IRE1α
pSEL-empty bait Empty
pMG1-UBD prey UBD
pMG1-empty prey Empty
pXP2d2-rPAP1 Luciferase STAT3 reporter plasmid
pMG1-REM2 RAS (RAD and GEM)-like GTP-binding 2 protein (REM2)